AUTOPHAGOSOME CONTENT PROFILING USING PROXIMITY BIOTINYLATION PROTEOMICS COUPLED TO PROTEASE DIGESTION IN MAMMALIAN CELLS

Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells

Summary: The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation.Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry, suitable to specifically map the Capacitor content of autophagosomes and potentially other short-liv

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Prediction of Recurrent Atrial Tachyarrhythmia After Receiving Atrial Flutter Ablation in Patients With Prior Cardiac Surgery for Valvular Heart Disease

Background: Surgical scars cause an intra-atrial conduction delay and anatomical obstacles that facilitate the perpetuation of atrial flutter (AFL).This study aimed to investigate the outcome and predictor of recurrent atrial tachyarrhythmia after catheter Alarm Clock ablation in patients with prior cardiac surgery for valvular heart disease (VHD)

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Determination of Fatty Acid Content of Rice during Storage Based on Feature Fusion of Olfactory Visualization Sensor Data and Near-Infrared Spectra

This study innovatively proposes a feature fusion technique to determine fatty acid content during rice storage.Firstly, a self-developed olfactory visualization sensor was used to capture the odor information of rice samples at different storage periods and a portable spectroscopy system was employed to collect the near-infrared (NIR) spectra duri

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